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Staphylococcal cell separation depends largely on the bifunctional autolysin Atl that is processed to amidase‐R1,2 and R3‐glucosaminidase. These murein hydrolases are targeted via repeat domains (R) to the septal region of the cell surface, thereby allowing localized peptidoglycan hydrolysis and separation of the dividing cells. Here we show that targeting of the amidase repeats is based on an exclusion...
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