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In this study, a semi-continuous culture mode was used for photo-hydrogen production by Rhodopseudomonas sp. nov. strain A7 and 3 key operating parameters (the interval feeding time, initial feeding time and the feeding volume ratio) were optimized to improve hydrogen production performance. The rate and yield of hydrogen production decreased from 19.7 ml-H2/l/h to 4.63 ml-H2/l/h with the interval...
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