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The naturally occurring proline-rich cyclic octapeptide stylissamide X is for the first time total synthesized in the present study by a two-step solid-phase/solution synthesis strategy using two different synthetic routes. In this study, all the amino acids are of L-configuration. The linear octapeptide was assembled by standard Fmoc solid-phase peptide synthesis (SPPS) and cyclization was carried...
Background PRISE2 is a new software tool for designing sequence-selective PCR primers and probes. To achieve high level of selectivity, PRISE2 allows the user to specify a collection of target sequences that the primers are supposed to amplify, as well as non-target sequences that should not be amplified. The program emphasizes primer selectivity on the 3’ end, which is crucial for selective amplification...
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