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Recent microscopy techniques use nanoparticles as contrast agents that assist in realizing super resolved imaging of the inspected sample. While the sample itself is not labelled, its optical properties and form are revealed from the agent's location and movement. Gold nanoparticles are frequently used for compound cellular imaging due to their plasmonic resonance that occurs in the visible regime...
We present a method to improve the localization precision of nanoparticles over Gaussian fitting by imposing an interference pattern on the Point-Spread-Function. Localization precision of 0.1nm for a single emitter was obtained.
We applied nonlinear optimization to convert a cannula into a high-resolution computational-fluorescence microscope. The microscope was used to image fluorescent microspheres, genetically-encoded mouse-brain slices, genetically-encoded and fluorescent-stained C. elegans nematodes (both living and dead animals).
Localization of a single fluorescent particle with sub-diffraction limit accuracy is a key merit in fluorescence microscopy. Implementation of nonlinear filtering algorithms prior the localization process can improve the localization accuracy of standard existing methods and also enable the localization of overlapping particles, allowing the use of increased fluorophore activation density, and thereby...
An all optical Viterbi decoder on a silicon chip was designed. The device is composed of an optical memory cell and two interfering input arms that use QPSK modulation to encode the incoming bits. The device has a shape of a ring resonator with a size of just a few micrometers and relies on the coupling of modes. Data processing of a few picoseconds is within the ability of the device.
A method for eliminating the unwanted terms in an on axis hologram is presented. In this method, free randomly distributed nanoparticles are in proximity to the object and their Brownian motion encodes the spatial features of the object in the recorded hologram. The nanoparticles are localized and a decoding pattern is calculated for each frame. This decoding pattern is then used to remove the reference...
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