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Early biochemical data showed that aminoacyl-tRNA synthetases often displayed species-specific recognition of tRNA. We compared the ability of purified Saccharomyces cerevisiae and Escherichia coli arginyl-tRNA synthetases to aminoacylate native and transcribed yeast tRNA Arg as well as E. coli tRNA Arg . The kinetic data revealed that yeast ArgRS could charge E...
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