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The newt, Cynops pyrrhogaster, exhibits physiological polyspermic fertilization, in which several sperm enter an egg before egg activation. An intracellular Ca 2+ increase occurs as a Ca 2+ wave at each sperm entry site in the polyspermic egg. Some Ca 2+ waves are preceded by a transient spike-like Ca 2+ increase, probably caused by a tryptic protease in the sperm acrosome...
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