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A novel signal amplification method based on methylene blue (MB) and nicking endonuclease (NEase) was developed for Hg2+ detection. Hairpin-shaped probe A (PA) contains a thiol group at the 5′ end and methylene blue (MB) tag at the 3′ end. A NEase recognition sequence was embedded into the loop portion of the PA. PA was firstly immobilized on the Au electrode by a self-assembly approach through Au–S...
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