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Scanning acoustic microscopy (SAM) is a well-accepted modality for forming quantitative 2D maps of acoustic properties of soft tissues at microscopic scales. In our SAM studies, the sample is raster-scanned (spatial step size of 2 μm) using a 250 MHz transducer resulting in a 3D RF data cube. Each RF signal is processed to obtain, for each spatial location, acoustic parameters, e.g., speed of sound...
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