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We developed selective injection of a single particle sensor into a specific cell by optically-regulated cell adhesion and membrane fusion of liposome for intracellular measurement. Fluorescence sensor encapsulated in fusogenic liposome is fabricated by spontaneous transfer method. The liposome is consists of neural lipids and spiropyran (SP). SP is a photochromic chemical and used to control the...
Optical adhesion control of a functional gel microtool, made of hydrophilic photo-crosslinkable resin, was developed for on-chip cell manipulation. The functional gel microtool used can be manipulated by optical tweezers and functionalized by spiropyran chromospheres, which is a photochromic polymer. Spiropyran chromospheres were used for temporal cell adhesion of the gel microtool. The adhesiveness...
In noncontact cell manipulation with optical tweezers, we need to use microtools to avoid damages to the cells by direct laser irradiation. When we manipulate complex structures such as microtools, a multi-beam optical tweezers which can manipulate multiple objects is suitable; however manipulation speed or trapping number are not so good. Therefore, we developed an integrated optical tweezers by...
In this study, we succeeded in producing functional microtool made of gel-microbead using size-dependent microparticle classification. Gel-microbead, which is used as the material for the microtool, is made by salting-out of hydrophilic photo-crosslinkable resin, ENT-3400. These gel-microbeads were separated according to their size by microfilters made of polydimethylsiloxane (PDMS). A first on is...
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