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A feeding trial was conducted to determine the optimum dietary protein requirement of a new Japanese strain of juvenile Chinese soft-shell turtle, Pelodiscus sinensis. Five iso-energetic diets were formulated to contain graded protein levels of 340, 370, 400, 430 or 460gkg −1 diet and fed to triplicate groups of turtles (mean initial weight, 3.70±0.05g) for eight weeks. The turtles were reared...
An improved method of slide preparation on special membrane was developed and a high quality of chromosome spreads was obtained from female half-smooth tongue sole (Cynoglossus semilaevis). The W chromosome was isolated and the sex-chromosome-specific painting probes were prepared by means of laser microdissection. The hybridization signals revealed by Cot-1 blocked and unblocked slides indicated...
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