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The level of synaptotagmin I or II in developing spinal neurons was increased by injection of synaptotagmin messenger RNA into early blastomeres of Xenopus embryos. The effect of overexpression of synaptotagmin on synaptic function was assayed in Xenopus nerve-muscle cultures within two days after injection. At neuromuscular synapses made by synaptotagmin-overexpressing neurons, the frequency of...
Mammalian brains contain a cdc2-like protein kinase which is a heterodimer of cyclin-dependent kinase 5 (Cdk5) and a brain-specific regulatory subunit with a molecular weight of 35,000, named p35 nck5a . Cdk5 has been identified as the major phosphorylating enzyme of tau protein in the brain. In this study, we examined the temporal and spatial expression patterns of p35...
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