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Previous studies on dorsal motor nucleus of the vagus (DMNV) neurons have mainly used in vivo animal models and in vitro brainstem slices. Primary culture of postnatal DMNV neurons in defined serum free medium has not been reported. We report a method for culture of postnatal rat DMNV neurons using serum free medium. Cultured DMNV neurons contain both Hu positive precursor cells and mature cells staining...
Sarosi, G. A. Jr., B. C. Kimball, D. C. Barnhart, W. Zhang and M. W. Mulholland. Tachykinin neuropeptide-evoked intracellular calcium transients in cultured guinea pig myenteric neurons. Peptides 19(1) 75-84, 1998.-Substance P and related tachykinins are present in the mammalian gut and act as neurotransmitters. Microfluorimetric measurement of intracellular calcium ([Ca 2+ ] i ...
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