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The binary toxin ‘Photorhabdus insect‐related’ proteins (PirAB) produced by Photorhabdus luminescens have been reported to possess both injectable and oral activities against a range of insects. Here, PirAB‐fusion protein was constructed by linking pirA and pirB genes with the flexible linker (Gly4Ser)3DNA encoding sequence and then efficiently expressed in Escherichia coli. To better understand the...
The enterobacterium Photorhabdus luminescens produces a number of toxins to kill its insect host. By analyzing the genomic sequence of P. luminescens TT01, we found that amino acid sequences encoded by plu1961 and plu1962 showed high similarity to XaxAB binary toxin of Xenorhabuds nematophila, which has both necrotic and apoptotic activities in both insect and mammalian cells in vitro. To evaluate...
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