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The toll‐like receptors (TLR) tlr22 was identified and characterized for the first time in one of the economically most important freshwater fish species in China, Megalobrama amblycephala. The full‐length cDNA (4039 bp) of M. amblycephala tlr22 contains an open reading frame of 2706 bp, encoding a 901 amino‐acid long polypeptide. The putative polypeptide contains 16 leucine‐rich repeat (LRR) motifs,...
The amplified fragment‐length polymorphism (AFLP) technique was used to identify sex‐specific markers in bluegill sunfish. A total of 12 835 loci were produced by 256 primer combinations, of which nine (0·73‰) exhibited presumed sex‐associated amplifications in the pooled samples; however, none of which revealed sex specificity upon individual evaluation.
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