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Resolution of the cis-, trans-isomers of vitamin K1 (phylloquinone) is important in nutritional analysis since only the trans-isomer is biologically active, while artificial vitamin K1 may contain significant amounts of the inactive cis-isomer. The cis-, trans-isomers of vitamin K1 were separated, with a resolution of 3.4, using pure carbon dioxide (CO2) as the mobile phase, with a run time ≈6 min,...
In a previous report, it was shown that the trans-isomers of the 2′–3′ double bond on the side chain of vitamin K1 (phylloquinone) represented 88.65 % of a specific sample, while the cis-isomer represented 11.35 %. However, the side chain contains 2 chiral centers, indicating there are 8 possible stereoisomers. Seven of these 8 stereoisomers were partially resolved in 20 min, on a RegisPack 4.6 × 250 mm,...
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