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The sinus node is an intensively researched structure in terms of anatomical, histological, electrophysiological, molecular and genetic approach. For postmortem diagnosis it is still difficult to investigate due to a still reduced accessibility. In this study we tried and succeed to apply molecular biology techniques on postmortem tissues in order to widen the range of postmortem forensic investigation...
In this study, a fixation protocol using a 10% neutral buffered formalin (FA) solution and another protocol using a methanol (MeOH) solution were compared for detection of ion channels, Kᵥ 1.5, Kᵥ 4.2, Caᵥ 1.2, Kᵢᵣ6.2, Naᵥ 1.5 and Naᵥ 1.1 in rat myocytes by immunolabelling. Kᵥ 1.5 and Kᵥ 4.2 at intercalated discs and Caᵥ 1.2 at transverse tubules were not detected by FA but were detected by MeOH....
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