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Capillary zone electrophoresis (CZE) was used to determine Hela cells activity with Hela treated by 0–46 μM methylmercury (MeHg) as the apoptosis model. The treated and untreated cells were stained by four different dyes (Janus Green B, Rhodamine 123, Neutral Red and Trypan Blue) and analyzed by CZE with UV/Vis detection. The absorbance of cells at 214 nm could indicate the degree of cell shrinkage...
We introduce here a method for continuous intact cell detection and viability determination of individual trypan blue stained cells by CE with ultraviolet–visible dual‐wavelength detection. To avoid cell aggregation or damage during electrophoresis, cells after staining were fixed with 4% formaldehyde and were continuously introduced into the capillary by EOF. The absorbance of a cell at 590 nm was...
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