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Prediction of B cell epitopes of duck enteritis virus (DEV) VP19c protein encoded by UL38 gene and expression in prokaryotic system were described in the present study. The major antigenic portion of VP19c protein of DEV was predicted and corresponding gene was amplified from the extracted DNA and cloned. The recombinant expression construction(pPAL7-truncated UL38) was identified by the polymerase...
In order to understand the relevant mechanism for biased usage of synonymous codons and the evolution and pathogenesis of DEV better, as well as for selecting appropriate expression systems to improve the expression of the target genes, we performed a comparative analysis of the codon usage bias in the UL47 gene of the duck enteritis virus (DEV) and the 26 reference herpesviruses. The results indicated...
The DPV UL46 gene( GenBank accession No. EU195108) isolated by our laboratory was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmid pMD18-T-UL46 was identified by PCR, restriction enzymes (BamHI+XhoI), sequencing and nucleic acid dot-hybridization. Then the gene was analyzed by bioinformatics tools. The results showed that a 2220bp complete open reading frame of 739 amino acids...
The UL27 open reading frame of the Duck Plague virus encodes glycoprotein B, the molecular characteristic of this gene was analyzed with bioinformatics software. The results indicated that this gene encodes an estimated 1000 putative proteins, contains the conserved domain of the Herpesvirus UL27-like protein. Similarity comparisons of DPV CHv strain gB protein sequences with those of 20 known herpesviruses...
Duck enteritis virus (DEV) is an alphaherpesvirinae that causes an acute, contagious, and fatal disease. In this study, the DEV-ULS3 gene from Key Laboratory of Animal Disease and Human Health of Sichuan Province was cloned and sequenced. Nucleotide sequence analysis revealed a 1032 bp open reading frame (ORF) theoretically encoding a 343-amino acid polypeptide was homologous to UL53 gene of alphaherpesvirinaes...
The focus of this paper is on the newly identified UL55 gene of DEV in our laboratory.Available bioinformatic tools were used to describe its molecular characteristics. A identified nucleotide sequence about 561bp of DEV UL55 ,consisting a complete ORF, was deduced to encode a protein with 186 amino acids.The 20.7981 kDa protein whose singnal peptide and transmembrane region are both absent. Codon...
The Us4 gene homologue of duck enteritis virus (DEV) was cloned by degenerate polymerase chain reaction (PCR) and sequenced. DEV Us4 gene open reading frame (ORF) was 1380 bp in length and its primary translation product was a polypeptide of 459 amino acids long. Phylogenetic tree of the nucleotide sequences showed this gene had a close evolutionary relationship with GaHV-1 and PsHV-1, but the DEV...
The analysis on codon usage bias of gI gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and molecular characteristic of DEV, and for selecting appropriate host expression systems to improve the expression of target gene. A comparative analysis of the codon usage bias of the DEV gI gene and 22 other refrence herpesviruses gI-like genes was performed, by using the...
A 831-bp complete open reading frame of the duck plague virus UL34 gene (GenBank accession no, EF643562) was identified in our laboratory. The molecular characteristic of this gene was amplified and analyzed with bioinformatics software. The results indicated that this gene encodes an estimated 276 putative protein, contains the conserved domain of the Herpesvirus-UL34-like protein. Phylogenetic tree...
The analysis on codon usage bias of UL26.5 gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and pathogenesis of DEV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the UL26.5 gene of DEV and 27 reference herpesviruses have been investigated . The...
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