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In this paper we present a hardware architecture with software implementation able to track free swimming single 2μm in diameter MC-1 bacterium. The computer vision system operates at up to 77 fps at full speed and up to 24 fps when recording full 512×512 pixels frame from coupled-charge device (CCD) array. Closed-loop control with lock-in tracking is achieved using the Otsu Segmentation Method (OSM)...
Computer cell tracking using an optical microscope and an image-processing module has proven to be a valuable technique for the study of cell behavior such as motility and migration. Validation of these techniques is difficult and often done by manual analysis or with the aid of limited and case-specific simulation software. We have developed a general simulation system that returns optical microscope...
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