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(3-aminopropl) triethoxysilaneand modified magnetic nanoparticles with the average diameter of 25.4nm were synthesized in water-phase co-precipitation method. And then these nanoparticles were covalently coupled with alkaline protease as enzyme carrier by using 1,4-phenylene diisothlocyanate as coupling agent. Experiments showed that the immobilized protease can keep the catalytic bioactivity, which...
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