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3-hydroxybutyrate (3HB), one of the degradation products of microbial biopolyesters polyhydroxyalkanoates (PHA), is a high energy metabolic substrate in animals. This study evaluated the effects of 3HB on growth of osteoblasts in vitro and on anti-osteoporosis in vivo. Alkaline phosphatase (ALP) assay, Van Kossa assay and Alizarin S red staining were used to study in vitro differentiation of murine...
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