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To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope with AV-PI staining was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4 times 10-5mol/L, 1.8 times...
To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice a tumor mouse model was developed, and the mice were treated with high, medium, and low doses of qinglongyi polysaccharide through abdominal injection for 7 d. Erythrocytes were collected and erythrocyte suspensions were prepared. Change of Ca2+ concentration in the erythrocytes of S180 mice was measured...
To investigate the effects of juglone on ROS production and mitochondrial transmembrane potential (DeltaPsim) in SGC-7901 cells in vitro, further to elucidate the apoptosis mechanism. The apoptosis morphological changes of SGC-7901 cells were observed using fluorescence microscope. The level of ROS and DeltaPsim of cells were detected by FCM. The change of [Ca2+]i in cells stained with Fluo-3/AM was...
To study the effect of growth inhibition and induction of apoptosis in sarcoma 180 cells by juglone in vivo. S180 tumor-bearing mice were set up to investigate the effects of juglone on tumor growth inhibition and apoptosis. After HE staining, the pathomorphologic changes of tumor cells were observed by optical microscope. The ultrastructural changes of tumor cells were observed by transmission electron...
This paper is to determine metabolites of toluene diisocyanate (TDI) in mice and deduced the pathway for toluene diisocyanate metabolism in the organism. Toluene diisocyanate was administered to mice for 2 weeks, samples of blood, feces, and urine were taken, and the structure of the toluene diisocyanate metabolites was determined using gas chromatography-mass spectrometry (GC-MS). Conditions for...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
To investigate the pro-apoptosis effect of isothiocyanates(ITCS) on human gastric adenoma cells SGC- 7901 and its mechanism. METHODS: SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential (Deltapsim),...
This study analyzed the effect of Asparagus polysaccharides on the migration time of erythrocytes in tumor- bearing organisms. A mouse tumor model was developed in which mice were intraperitoneally injected with high, medium, and low dosages of Asparagus polysaccharides for 7 d. The erythrocytes were collected and prepared into suspensions, and the migration time of cells were measured using high...
This paper is to study the effects of TDI on mice marrow cells . The effects of TDI were studied by laser scanning confocal microscope to investigate morphologic changing of the mice bone marrow cell and genotoxicity test -chromosomal aberration test and sister chromatic exchange (SCE) test. Results showed that the 1/16LC50 and 1/8LC50 dosage of TDI had no significant effects on the fluorescence pixels...
To study the effects of two kinds of cactus polysaccharides on band 3 protein content, cross-linking protein content, and the lipid fluidity of erythrocyte membrane in S180 mice. Membrane protein content is analyzed by SDS-PAGE, and lipid fluidity of the membrane is measured by the Skinitzky method. The two kinds of cactus polysaccharide increase the content of band 3 protein, decrease the content...
To observe the effect of solanine on the contents of caspase-3 and Bcl-2 in HepG2 , and to explicate the mechanism by which solanine induces the apoptosis of tumor cells.Laser confocal scanning microscopy (LCSM) and Western blot were used to measure the contents of caspase-3 and Bcl-2 , and LCSM was used to determine their locations in the cell. Solanine markedly increased the content of caspase-3...
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