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Using immunohistology with two specific antisera raised against N-terminal parathyroid hormone-related protein (PTHrP) and in situ hybridization (riboprobe to common coding exon), evidence is provided for the expression of PTHrP by mouse, rabbit, and human osteoclasts derived from several in vitro and in vivo sources. In cocultures of mouse bone marrow and calvarial cells treated with 1,25-dihydroxyvitamin...
Expression of parathyroid hormone-related protein (PTHrP) messenger RNA (mRNA) and protein was investigated throughout the developmental progression of endochondral bone formation in mouse and intramembranous bone formation in an in vivo model in rabbit, using in situ hybridization and immunohistochemistry. Endochondral bone formation was investigated in a developing embryo, newborn, and adult mouse...
Stromal cell-derived cytokines such as IL-11, which utilize the gp130 signal transducer may be pivotal in osteoclast development. To study the regulation of IL-11 production by primary murine osteoblasts, we used a new bioassay employing transfected Ba/F3 cells which expressed high affinity IL-11 receptors. Recombinant human IL-11 specifically stimulated proliferation of these cells in a dose dependent...
Interleukin-11 (IL-11) is a stromal cell derived cytokine which may be important for osteoclast development triggered by 1,25(OH)2D3 and other osteotropic factors. We prepared osteoclast-like multinucleated cells (OCL's) by maintaining cultures of murine bone marrow cells, or co-cultures of bone marrow and primary osteoblastic cells in the presence of 10nM 1,25 (OH)2D3 for up to 10 days. In order...
During embryogenesis, the creation of marrow sinusoids is intimately related to the coupled processes of osteogenesis and osteoclastic resorption. We set out to further define the relationship between bone formation and marrow development by implanting an intraosseous titanium device into the tibiae of rabbits which permits the examination of bone formation under standardized and reproducible conditions...
The influence of TGFβ 1 on bone was studied in a titanium device implanted into the tibia of rabbits. TGFβ 1 was infused via an Alzet osmotic pump calibrated to deliver at a rated of 200ng daily for 2 weeks before replacement. A hollow channel is incorporated into the device into which tissue can grow, and the histological sequence of events was observed over 6 weeks. In control...
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