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Summary The technique of quantitative competitive RT-PCR to determine the levels of mRNA expression of genes encoding peroxisome proliferator-activated receptor alpha (PPARα), acyl coenzyme-A oxidase (ACOX) and cytochrome P450 4A1 (CYP4A1) in primary rat hepatocyte cultures is described. This technique is based on the co-amplification of an internal standard (PCR MIMIC) and target DNA sequence with...
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