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To investigate transcription of pituitary glycoprotein hormone a and gonadotropin IIβ (GTH IIβ) subunits in female Japanese eel, gene quantitation systems using reverse transcription-polymerase chain reaction (RT-PCR) were developed, using β-actin as an internal control gene. These systems were not only sensitive, but also precise, with low coefficients of variation within and between assays (below...
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