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An affinity based bio-sensing technique was developed using an anti-transmissible spongiform encephalopathy monoclonal antibody to detect prion in 0.1mol/l sodium phosphate buffer. Fluoresein iso-thio-cyanate (FITC) labeled with a prion epitope (QYQRES) was used as a decoy for prions. Lowest detectable prion concentration was 8nmol/l in phosphate buffer. The bio-sensing scheme was used to probe the...
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