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For the sake of evaluating the effect of the hydrophobic residues insertion in the N-terminus of Staphylococcus simulans lipase (SSL), four residues of Isoleucines have been inserted at the N-terminus of this enzyme. The recombinant Staphylococcus simulans lipase (r-SSL) and its constructed mutant (4Ile-SSL) were expressed in Escherichia coli BL21 (DE3) and purified to homogeneity using classical...
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