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The addition of bradykinin to 32 P i -labeled neuroblastoma x glioma hybrid NG108-15 cells caused a substantial loss of radioactivity from phosphatidylinositol 4,5-bisphosphate (PI-4,5-P 2 ). The bradykinin-induced hydrolysis of PI-4,5-P 2 was almost equally observed even when extracellular Ca 2+ was depleted with EGTA (100μM). On the other hand, high...
Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma x glioma hybrid NG108-15 cells [(1984) J. Biol. Chem. 259, 10201-10207] using [ 3 H]inositol-labelled cells. Bradykinin produced net increases in the level of [ 3 H]inositol phosphates, especially of [ 3 H]inositol trisphosphate...
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