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A sensitive fluoroimmunoassay (FIA) was developed for the determination of human chorionic gonadotropin (β-HCG). It is based on fluorescent polymer nanoparticles (PFNPs) coated with anti-β-HCG monoclonal antibodies in a sandwich type of fluoroimmunoassay. The PFNPs were synthesized by precipitation polymerization using methacrylic acid (MAA) as the monomer, trimethylolpropane trimethacrylate as the...
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