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Incubation of GalNAcβ(1-4)GlcNAcβ-OMe with GDP-Fuc in the presence of human milk α34-fucosyltransferase resulted in the formation of GalNAcβ(1-4)[Fucα(1-3)]GlcNAcβ-OMe. Under conditions that led to complete α3-fucosylation of Galβ(1-4)GlcNAcβ-OEt, GalNAcβ(1-4)GlcNAcβ-OMe was fucosylated for more than 85%. For the identification of the isolated fucosylated products one- and two- dimensional 1 ...
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