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As sentinel species, earthworms such as Eisenia spp. and Lumbricus spp. have been considered among the best bioindicators or biomonitors for soil ecosystems owing to their close contact with the environment and essential roles in soil pedogenesis, structure, fertility and the terrestrial food chain. Earthworms have also been used extensively for assessing environmental risk and chemical toxicity in...
A physical disruption method was developed to extract ergosterol from soil samples. The optimised procedure was to extract 4g of moist soil with 6ml of methanol by shaking for 1h at 320rpm in the presence of 4g of glass beads. Approximately 102% of the spiked pure ergosterol (1, 2 and 30μgg -1 soil) was recovered from nine agricultural soils using this method. As a biomarker of fungal...
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