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GlcNAcβ1-2Man and GlcNAcβ1-6Man were synthesized using the reverse hydrolysis activity of β-N-acetylglucosaminidase from both jack beans and Bacillus circulans. In turn, Galβ1-4GlcNAcβ1-2Man and Galβ1-4GlcNAcβ1-6Man were synthesized regioselectively using the transglycosylation activity of β-galactosidase from Diplococcus pneumoniae and B. circulans, respectively. These di- and trisaccharides are...
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