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Biodegradable organic matter (BOM) in polluted water plays a key role in various biological purification technologies. The five-day biochemical oxygen demand (BOD5) index is often used to determine the amount of BOM. However, standard BOD5 assays, centering on dissolved oxygen detection, have long testing times and often show severe deviation (error ≥ 15%). In the present study, the coulombic yield...
Fluoroquinolones (FQs) are a group of antimicrobial agents that have been widely used for therapeutic purposes in clinical medicine for human and veterinary diseases, as well as in aquaculture production. Their residues, however, may survive in foods of animal origin, thus causing health risks for human. In this study, a rapid and sensitive method based on in vivo solid-phase microextraction (SPME)...
Tetrodotoxin (TTX) is one of the most toxic substances of non-protein in nature. In present study, a rapid and sensitive method based on in vivo solid-phase microextraction (SPME) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed to detect the endogenous TTX in pufferfish (Fugu). Fiber evaluation experiments demonstrated that, compared with the commercial PDMS and...
The concentration of five rapidly metabolized anesthetics in living tilapias was determined in this study, by the presented method coupling in vivo solid phase microextraction (SPME) to gas chromatography-mass spectrometry (GC-MS), which was the first time that in vivo sampling method was adapted in detecting the anesthetic residue in the living aquatic product. The analytical performance of the developed...
Immunoassay for cancer biomarkers plays an important role in cancer prevention and early diagnosis. To the development of immunoassay, the quality and stability of applied antibody is one of the key points to obtain reliability and high sensitivity for immunoassay. The main purpose of this study was to develop a novel immunoassay for ultrasensitive detection of cancer biomarker alpha-fetoprotein (AFP)...
In this study, a rapid and accurate determination strategy was established for simultaneous measurement of melamine (MLM) and dicyandiamide (DCD) directly in powdered milk by coupling molecularly imprinted solid-phase extraction (MISPE) with high performance liquid chromatography (HPLC). A novel double-template technique was adopted for preparing SPE packing agent and the obtained double-templated...
Spectrophotometric determination of procaine hydrochloride is described. The procaine hydrochloride reacts with p-dimethylaminobenzalhyde in glacial acetic acid to form an Schiff base which is a yellow compound, and its maximum absorption wavelength is at 455nm, 455 =3.46x10 4 . The absorbance for procaine hydrochloride from 0.2 to 15 μg ml -1 obeys Beer's law...
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