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The immobilization of proteins was performed by ionic adsorption on agarose supports coated with polyethyleneimine of high molecular size (PEI) (600kDa). The immobilization processes were carried out at different ionic strengths (5, 50, 100, 300, 500 and 700mM NaCl). At NaCl concentrations lower than 100mM, the immobilization proceeded very rapidly in all cases, while in some cases at concentrations...
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