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Porcine pancreatic lipase extract has been used as a biocatalyst for many years, however it is a rather complex mixture with various active enzymes. In this work, we have developed a protocol to purify three different enzymes with esterase activity and lipase properties: the actual Porcine pancreatic lipase; a previously reported 33kDa lipase and a third one with a molecular weight of 25kDa. They...
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