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An integrating plasmid pMGI6 carrying glucoamylase gene (GLA) and using the yeast α-acetolactate synthase gene (ILV2) as the recombination sequence, was constructed from pBluescript II SK. Theilv2∶∶GLA fragment released from pMGI6 was introduced into the brewing yeastSaccharomyces pastorianus and the resulting recombinant strain was able to utilise starch as the sole carbon source, its glucoamylase...
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