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Here, we report biomodification of temperature-responsive culture surfaces with biotinylated biomolecules utilizing streptavidin and biotinylation of the surfaces. Poly(N-isopropylacrylamide-co-2-carboxyisopropylacrylamide) was covalently grafted onto tissue culture polystyrene (TCPS) dishes. Biotinylated Arg-Gly-Asp-Ser (RGDS) peptides with different spacer lengths (biotin-conjugated G n ...
This paper describes a novel method to immobilize biomolecules such as growth factors and enzymes onto the surface of poly (N-isopropylacrylamide-co-2-carboxyisopropyl-acrylamide (poly(IPAAm-co-CIPAAm))-grafted tissue culture polystyrene dish (TCPS) via affinity binding between avidin and biotin. Effects of Arg-Gly-Asp-Ser (RGDS) peptides with different spacer length on the behavior of cell attachment/detachment...
Photopolymerization has been widely used for surface micropatterning. The technique often requires photomasks and light sources with appropriate energies or filters. For rapid prototyping of surface photo-micropatterning, we have developed a novel device by modifying a commercially available liquid crystal device projector. In place of the image expansion unit of the projector, we attached an image...
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