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An oil spill accident happened to a Russian tanker Nakhodka with a cargo of heavy oil type C in the Sea of Japan on January, 1997 and the spilled oil severely polluted the coast of Japan and damaged the environment. In this study, androgenic and antiandrogenic activities of C-heavy oil crude extracts prepared with ethanol were evaluated on two androgen-responsive cell lines, a Shionogi mouse mammary...
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