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A new method for generation and screening Chinese cabbage mutants was employed in this study. In order to quickly obtain homozygous mutants in Chinese cabbage, isolated microspore culturing and ethyl methanesulfonate mutagenesis were performed on a doubled haploid line. Microspores were treated with different concentrations (0.04, 0.08, 0.12 %, v/v) of ethyl methanesulfonate solution for 10 min, and...
Photosynthesis of carbohydrates is the primary source of grain yield in wheat. Photosynthetic organs, especially flag leaves and awns play important roles in wheat growth and development. Genetic analysis of flag leaf posture, size and shape and presence/absence of awns was conducted using a set of 269 recombinant inbred lines (RILs) derived from Yanda1817 × Beinong6. Six agronomic traits comprising...
Chinese cabbage (Brassica rapa L. ssp. pekinensis) is one of the most important vegetables in China. However, the inheritance of yield-related traits in Chinese cabbage is poorly understood to date. To map quantitative trait loci (QTL) for yield-related traits in Chinese cabbage, a genetic linkage map was constructed with 192 doubled haploid (DH) lines. The genetic map was constructed based on 190...
Leaf rust, caused by Puccinia triticina, is an important disease for wheat production, both in China and worldwide. In laboratory studies spelt wheat (Triticum aestivum ssp. spelta) landrace Altgold was resistant to P. triticina races THT and PHT and genetic analysis indicated that it possessed a dominant leaf rust resistance gene, temporarily designated LrAlt. F6 recombinant inbred lines (RILs) derived...
Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease of wheat (Triticum aestivum) in China and worldwide, causing severe yield losses annually. Wild emmer (T. dicoccoides) accession IW72 collected from Israel is resistant to powdery mildew at the seedling and adult stages. Genetic analysis indicated that the resistance was controlled by a single dominant gene,...
Pm12, transferred from Aegilops speltoides (2n = 2x = 14, genome SS) to wheat, confers effective resistance to powdery mildew worldwide. By applying bulked segregant analysis in a BC3F2 segregating population consisting of 305 plants, 18 wheat genomic and EST-SSR markers linked to the resistance gene were identified. Pm12 was located in the 6SS portion of the T6BS-6SS.6SL translocation chromosome...
The leaf and stem surfaces of many land plants are covered with a cuticular wax layer that confers a glaucous appearance or white bloom. Synthetic hexaploid wheat Line 3672 was non-glaucous, and common wheat Line 9753 was glaucous. The cuticular wax was characterized using Scanning Electron Microscopy (SEM). A hybrid using 9753 as female parent and 3672 as male parent was made and 108 F2 plants and...
Powdery mildew caused by Erysiphe graminis f. sp. tritici is one of the most important wheat diseases in many regions of theworld. A powdery mildew resistance gene, originating from wild emmerwheat (Triticum dicoccoides) accession `C20', from Rosh Pinna, Israel,was successfully transferred to hexaploid wheat through crossing andbackcrossing. Genetic analysis indicated that a single dominant genecontrols...
Differential display of mRNA was used to analyze the differences of gene expression in seedling leaves between heterotic hybrid/nonheterotic hybrid and their parental inbreds in order to study the molecular basis of heterosis in wheat. The results indicated that patterns of gene expression in hybrids differ significantly from their parents. Both quantitative and qualitative differences were observed...
An endemic hexaploid wheat found in Tibet, China was taxonomically classified as a subspecies in common wheat, i.e. Triticum aestivum ssp. tibetanum. Seven accessions of the Tibetan wheat, 22 cultivars of common wheat and 17 lines of spelt wheat were used for RAPD analysis to study the genetic relationships of the Tibetan wheat with common wheat and spelt wheat, and to assess the genetic diversity...
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