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In this study, a fast and sensitive sandwich assay has been developed with the combination of immunomagnetic separation (IMS) and fluorescence techniques to enumerate Escherichia coli (E.coli). Iron oxide core gold shell (Fe3O4@Au) magnetic nanoparticles were prepared and modified with biotinylated antibodies specific to E.coli. Fluorescence labels have been constructed by preparing chitosan coated...
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