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Extraction of dry bacteria of Acinetobacter baumannii strain 24 by phenol-water yielded a lipopolysaccharide (LPS) that was studied by serological methods and fatty acid analysis. After immunisation of BALB/c mice with this strain, monoclonal antibody S48-3-13 (IgG 3 isotype) was obtained, which reacted with the LPS in western blot and characterized it as S-form LPS. Degradation of the LPS...
The lipopolysaccharide from Klebsiella oxytoca rough mutant R29 (O1 - /K29 - ) has been isolated and its complete structure has been elucidated by compositional analyses, NMR spectroscopy, and laser-desorption mass spectrometry. The carbohydrate backbone has the structure of which the GlcN residues (the lipid A backbone) are acylated by 14:(3-OH) (amide-linked) and 12:0, 14:0(3-OH)(ester-linked)...
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