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Objective To investigate the specific blockage effect of individual antisense RNA on mutant p53 gene in vitro. Methods The single strand antisense transcription system containing mt-p53 exon 8 sequence (pGEM3zf(+/-)p53exon8) was constructed. The ligation of antisense RNA with mt-p53 gene was confirmed by in situ hybridization; MDA-MB-231 human breast cancer cells were transfected with ASp53exon8’RNA...
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