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Three new C-3 dehydrated bufadienolides were isolated from the venom of Bufo bufo gargarizans. Their structures were elucidated as 5β,12β-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide (1), 5β,12β-12,14-dihydroxy-11-oxobufa-2,20,22-trienolide (2), and 5β,12α-12,14-dihydroxy-11-oxobufa-2,20,22-trienolide (3) on the basis of extensive spectroscopic analysis, especially 1D NMR and 2D NMR data. In addition,...
Six new bufadienolides (1–6) and two new C 23 steroids (7 and 8), together with three known compounds (9–11) were isolated from the venom of Bufo bufo gargarizans. Their structures were elucidated by spectroscopic analysis and single-crystal X-ray diffraction. In vitro cytotoxicities of all compounds were evaluated in A549 cancer cell line. Compounds 2, 3 and 10 showed significant cytotoxic...
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