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The codon usage bias of DPV UL19 gene and 25 reference herpesviruses have been analyzed in this study. This work was performed by program CodonW 1.4, and CUSP and CHIPS of EMBOSS. The results indicated that codons of DPV UL19 gene were strong bias towards the synonymous codons with A or T at the third codon position. The codon adaptation index (CAI), effective number of codons (ENC), and GC3s values...
In the study, we calculated the codon usage bias in the newly identified UL38 gene of Duck Enteritis Virus (DEV) and performed a correlation analysis of codon bias of DEV UL38 gene and its host animal. There was a strong bias in the DEV UL38 gene towards the synonymous codons with A and T at the third codon position. The amino acids codon usage bias in the DEV UL38 gene was compared with other 15...
Glycoprotein K encoded by the DEV-UL53 gene is one of the DEV envelope glycoproteins. Like most members of the alphaherpesvirinae, the genes in the UL region of its genome are well conserved, which means that the major DEV envelope glycoprotein K possessing similar functions of envelope glycoprotein K of alphaherpesvirinae plays an essential role in viral replication and cell fusion. Glycoprotein...
In order to understand the relevant mechanism for biased usage of synonymous codons and the evolution and pathogenesis of DEV better, as well as for selecting appropriate expression systems to improve the expression of the target genes, we performed a comparative analysis of the codon usage bias in the UL47 gene of the duck enteritis virus (DEV) and the 26 reference herpesviruses. The results indicated...
The analysis on codon usage bias of Us4 gene of Duck Plague Virus (DPV) may provide a basis for understanding the evolution and pathogenesis of DPV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the Us4 gene of DPV and 17 reference herpesviruses have been investigated . The results reveals...
The DPV UL46 gene( GenBank accession No. EU195108) isolated by our laboratory was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmid pMD18-T-UL46 was identified by PCR, restriction enzymes (BamHI+XhoI), sequencing and nucleic acid dot-hybridization. Then the gene was analyzed by bioinformatics tools. The results showed that a 2220bp complete open reading frame of 739 amino acids...
Abstract-An open reading frame (ORF) containing 675 nucleotides was got by sequencing the DEV gene libraries constructed by our laboratory. The ORF was identified as DEV UL45 gene by aligning with gene bank database using the software of BlastN and ORF Finder. Specific primers were designed and PCR products containing this ORF were cloned into the vector of PMD18-T. Dot blot confirmed the UL45 gene...
The UL27 open reading frame of the Duck Plague virus encodes glycoprotein B, the molecular characteristic of this gene was analyzed with bioinformatics software. The results indicated that this gene encodes an estimated 1000 putative proteins, contains the conserved domain of the Herpesvirus UL27-like protein. Similarity comparisons of DPV CHv strain gB protein sequences with those of 20 known herpesviruses...
The focus of this paper is on the newly identified UL55 gene of DEV in our laboratory.Available bioinformatic tools were used to describe its molecular characteristics. A identified nucleotide sequence about 561bp of DEV UL55 ,consisting a complete ORF, was deduced to encode a protein with 186 amino acids.The 20.7981 kDa protein whose singnal peptide and transmembrane region are both absent. Codon...
The analysis on codon usage bias of gI gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and molecular characteristic of DEV, and for selecting appropriate host expression systems to improve the expression of target gene. A comparative analysis of the codon usage bias of the DEV gI gene and 22 other refrence herpesviruses gI-like genes was performed, by using the...
A 831-bp complete open reading frame of the duck plague virus UL34 gene (GenBank accession no, EF643562) was identified in our laboratory. The molecular characteristic of this gene was amplified and analyzed with bioinformatics software. The results indicated that this gene encodes an estimated 276 putative protein, contains the conserved domain of the Herpesvirus-UL34-like protein. Phylogenetic tree...
The Duck Plague Virus (DPV) gE gene was identified by constructing the DPV genomic library, and we analyzed the codon usage of DPV gE gene by using CAI, CHIPS and CUSP program of EMBOSS. The results showed that codon usage bias in the DPV gE gene was strong bias towards the synonymous codons with A and T at the third codon position. The amino acids codon usage bias in the DPV gE gene was compared...
The analysis on codon usage bias of UL26.5 gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and pathogenesis of DEV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the UL26.5 gene of DEV and 27 reference herpesviruses have been investigated . The...
A recombinant plasmid selected from DEV gene libraries constructed by our lab was sequenced and an open reading frame (ORF) containing 1398bp nucleotides was preliminarily identified by aligning with gene bank database by the software of BlastN and ORF Finder. Specific primers were designed and PCR products containing this ORF were cloned into the vector of PMD18-T. DNA sequence analysis showed that...
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