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The aim was to identify the codon usage bias between the newly identified Riemerella Anatipestifer(RA)outer membrane efflux protein gene(GenBank accession NO. CP003388) and outer membrane efflux protein genes of 17 other reference flavobacteriaceae with bioinformatics software. A comparative analysis of the codon usage bias of the 18 flavobacteriaceae was performed by using the CodonW1.4.2 and CUSP...
Object: The study herein was aimed to provide a comprehensive analysis of synonymous codon usage in Riemerella anatipestifer (RA) Cas1 gene and a comparative study of the codon usage bias between RA and other 11 reference strains within the Flavobacteriaceae family. Methods: Using bioinformatics method, the values of relative synonymous codon usage (RSCU), the effective number of codons (ENc), G+C...
The bioinformatics analysis of D15 gene, encoding the 99-kDa major surface antigen D15 of Riemerella anatipestifer, the etiological agent of septicemia anserum exsudativa, was first provided in this article. The program DNAStar, ORF Finder, CDD, ClustalX, MEGA, NetNGlyc, NetOGlyc as well as NetPhos were performed to predict the characteristic of the D15 gene in physicochemical, construction of phylogenetic...
The analysis on codon usage bias of major facilitator superfamily mfs_1 gene of Riemerella anatipestifer (RA) may improve our understanding of the evolution and pathogenesis of RA and provide a basis for understanding the relevant mechanism for biased usage of synonymous codons and for selecting appropriate expression systems to improve the expression of target genes. All of these analysis results...
D15 protein which encoded by the RA-D15 gene was one of the Riemerella anatipestifer's surface antigens. The precursor RA-D15 protein was encoded by 859 amino acid residues, which was 99-kDa in theoretical molecular weight. The putative protein had no signal peptide, 32 potential antigenic determinants. RA-D15 protein was a type membrane protein that could be divided into 3 distinct functional domains:...
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