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Three types of bacteria were immobilized by the calcium alginate gel occlusion method, and a microbial rod using the immobilized microbial membrane was prepared for the determination of glucose. The hydrogen peroxide produced from glucose by microbial enzyme action at pH 7.2 was measured by use of oxidative coupling of 4-chlorophenol and 4-aminoantipyrine catalyzed by mimetic peroxidase (manganese[5,10,15,20-tetrakis(4-sulphophenyl)-21H,23H-porphine])...
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