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New type of copolymers of propylene oxide and ethylene oxide was assessed for promoting delivery of plasmid DNA based gene therapeutics. Lipid-like trifunctional copolymers (TFCs), with both random or diblock structures and relatively low hydrophilic–lipophilic balance, were studied and compared with linear Pluronic™ L61. Structure-dependent relationships for micelle-forming, cytotoxic and hemolytic...
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