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A genomic DNA segment and cDNAs encoding an extracellular exoinulinase fromAspergillus niger strain 12 were cloned and sequenced. Southern blot analysis indicated that the exoinulinase gene (inuE) was present as a single copy in the genome. An open reading frame of 1611 by was interrupted by a single intron of 60 bp, and encoded a 19-amino acid signal peptide and a 518-amino acid mature protein. The...
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