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This paper discussed the lethal effects of steep pulsed electric field (SPEF) to target lymphatic capillaries in VX2 implanted breast cancer of rabbits using methylene blue dye injection staining, 5'-AMP-ALPase enzyme histochemical double staining, electron microscopy and VEGF-C observations. The result of methylene blue dye perfusion showed that the edge of tumor treated with SPEF was indistinct,...
Exposing cancer cell to steep pulsed electric field (SPEF) with appropriate dosage, the cell may die due to irreversible electrical breakdown (IREB). 48 BALB/c mice inoculated with U/sub 14/ cervical cancer cell were treated with SPEF to study the lethal and inhibitory effects of SPEF on them. They were randomly divided into test group (26) and control group (22), and then four experiments were carried...
The vascular effect and immunity effect of SPEF on Wistar mice were studied, and the possible treatment mechanisms and clinical trial methods of SPEF were discussed. We tested lymphocyte proliferation ratio and activity of NK cell, and the contents of IFN-/spl alpha/ and IL-4 in the serum lymphocyte were inspected with ELISA method. Moreover, macrophage were collected, the content of TNF-/spl alpha/...
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