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A novel cell separation process based on immunoaffinity aqueous two phase systems is presented to isolate and purify CD34+ stem/progenitor cells directly from the whole umbilical cord blood (UCB). A system, composed of polyethylene glycol and dextran, was evaluated for the selective recovery of CD34+ cells from UCB. A monoclonal antibody against the CD34 surface antigen was used for the direct partitioning...
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